this post was submitted on 31 May 2024
415 points (96.4% liked)
Science Memes
10988 readers
1894 users here now
Welcome to c/science_memes @ Mander.xyz!
A place for majestic STEMLORD peacocking, as well as memes about the realities of working in a lab.
Rules
- Don't throw mud. Behave like an intellectual and remember the human.
- Keep it rooted (on topic).
- No spam.
- Infographics welcome, get schooled.
This is a science community. We use the Dawkins definition of meme.
Research Committee
Other Mander Communities
Science and Research
Biology and Life Sciences
- !abiogenesis@mander.xyz
- !animal-behavior@mander.xyz
- !anthropology@mander.xyz
- !arachnology@mander.xyz
- !balconygardening@slrpnk.net
- !biodiversity@mander.xyz
- !biology@mander.xyz
- !biophysics@mander.xyz
- !botany@mander.xyz
- !ecology@mander.xyz
- !entomology@mander.xyz
- !fermentation@mander.xyz
- !herpetology@mander.xyz
- !houseplants@mander.xyz
- !medicine@mander.xyz
- !microscopy@mander.xyz
- !mycology@mander.xyz
- !nudibranchs@mander.xyz
- !nutrition@mander.xyz
- !palaeoecology@mander.xyz
- !palaeontology@mander.xyz
- !photosynthesis@mander.xyz
- !plantid@mander.xyz
- !plants@mander.xyz
- !reptiles and amphibians@mander.xyz
Physical Sciences
- !astronomy@mander.xyz
- !chemistry@mander.xyz
- !earthscience@mander.xyz
- !geography@mander.xyz
- !geospatial@mander.xyz
- !nuclear@mander.xyz
- !physics@mander.xyz
- !quantum-computing@mander.xyz
- !spectroscopy@mander.xyz
Humanities and Social Sciences
Practical and Applied Sciences
- !exercise-and sports-science@mander.xyz
- !gardening@mander.xyz
- !self sufficiency@mander.xyz
- !soilscience@slrpnk.net
- !terrariums@mander.xyz
- !timelapse@mander.xyz
Memes
Miscellaneous
founded 2 years ago
MODERATORS
you are viewing a single comment's thread
view the rest of the comments
view the rest of the comments
Result?
We just tried to find the quantity of curcuminoids in curcuma if it was boiled before being turned into powder compared to not being boiled.
We tried to use the HPLC, but it seems that our samplesclogged the column. So we failed ๐ .
For our abstract, we did look at other studies on the supposed benefits of curcuma, but none of them were rigorous enough to say that there is a benefit (for example, one study informed the people being tested if they got curcuma or anotherantiinflammatory medicine, which already biases the results)
did you unclog the column or does lab has to eat up few thousand dollar loss
We unclogged the column, but since it was clogging it up for each sample and it's quite damaging to the column, we decided to stop. Our budget was quite tight, since this was a school project.
jeez dude are you using some pretreatment
Idk what pretreatment really entails, but we did dilute it, if that counts ๐
All HPLC samples that i do, before putting them on column i filter them through short layer of silica to separate all the insoluble and entirely too polar crap
Additionally there's also short guard column before main column, so if something goes really fucking wrong it's the 2cm guard column that is to be replaced and not the 20cm main one
I work mostly with NP-HPLC so your situation might be a bit different, but the general principle is there