this post was submitted on 13 Jun 2023
50 points (100.0% liked)

Science

13017 readers
38 users here now

Studies, research findings, and interesting tidbits from the ever-expanding scientific world.

Subcommunities on Beehaw:


Be sure to also check out these other Fediverse science communities:


This community's icon was made by Aaron Schneider, under the CC-BY-NC-SA 4.0 license.

founded 2 years ago
MODERATORS
you are viewing a single comment's thread
view the rest of the comments
[โ€“] appel@whiskers.bim.boats 7 points 1 year ago* (last edited 1 year ago) (1 children)

I wanted to check which cells they used, because using bacteria would give it no power at all, as bacteria have very different uptake, DNA damage tolerance and DNA repair mechanisms. they used:

  • SW620: a epithelial cancer cell line from a 51 yr old male's colorectal cancer
  • HEK293 an immortalised cell line from a human embryo kidney
  • MCF-7 a breast cancer cell line.
  • HeLa the infamous HPV cervical cancer cell line.

source

So they are all human cells, and the SW620 cells would be somewhat similar to our gut epithelial cells, as they once were the same. It's hard to be certain though, because immortal cell lines can accumulate many differences since they were isolated.

The SW620 cells did take up the damaged nucleosides, and more so than HeLa or MCF-7.

[โ€“] sneezycat@sopuli.xyz 3 points 1 year ago* (last edited 1 year ago)

Thanks for the clarification! I definitely should've read the actual paper before commenting :)

I edited my comment to reflect this